Terre Des Dames has been certified organic for over ten years. The structure of the vineyard is unchanged from 200 years ago. The 25 small plots (totalling 14 hectares) are on different levels, surrounded by low walls and diverse garrigue. No pesticides or artificial fertilizers are used. Most of the year, natural grass growth between the rows supports the life of the soil. The microclimate of the area, along with the underground springs and rivers, imparts freshness to the vine roots. As well, the roots are nourished and protected by large barriers of trees and shrubs between the plots. Maintaining this biodiversity protects against disease. The soil is clay, limestone, and flint and is rich with marine fossils. Underground streams counter the arid environment, flowing beneath the estate and irrigating the vines. An emphasis is placed on preserving the old vines as their deep roots are resistant to global warming. The domaine's oldest vine is 100 years old and other vines are between 50 and 70 years old.